PCR:
-can amplify any piece of DNA without using cells like Sanger-Heat is used to denature DNA
-Errors of PCR limits the number of good copies that can be made when large amount of gene are needed.
-DNA is incubated in a test tube with special DNA polymerase (Taq pol)
-DNA template, dNTP, primers (2 known sequence), and taq pol
-has repetitive steps of heating and then cooling DNA
-used in RFLP
Vector Cloning:
-Apply gene of interest into plasmid to bacterial cell
-DNA ligase glue cut sequence of DNA together
-Direct manipulation of genes for practical purposes
-gel electrophoresis and RFLP used to contain gene of interest
-uses RE, vector DNA, and bacterial cells
-Takes longer than the other methods
Sanger DNA sequencing:
-can amplify any piece of DNA without using cells like PCR
-DNA template, dNTP, primers (1 known sequence), polymerase, and ddNTP
-the first letter of the new sequence is missing
-used to sequence DNA
All:
-all need DNA template
-all produce new copies of DNA
-all manipulate DNA
